Chemical & Clinical

iCinac L.A.B. Fermentation Monitor Series

iCinac L.A.B. Fermentation Monitor Series
iCinac L.A.B. Fermentation Monitor Series

Product Overview

Lactic Acid Bacteria Fermentation Kinetics Monitoring and Reactor Control

The main function of Lactic Acid Bacteria (L.A.B.) is to produce Lactic Acid, which is the acidification process of foodstuffs. The main application of L.A.B. is as starter cultures in the food industry with an enormous variety of fermented products – dairy, meat, fish, fruit, vegetables and cereal products.

The AMS Alliance iCinac Series is heavily used by ferment and starter culture manufacturers, both for product quality control and development of new cultures for the dairy industry. Monitoring the behavior of these starters allows you to optimize these difficult processes, ensuring consistent L.A.B. activity and consistent quality of the end product.

The iCinac Series is a unique solution for kinetic analysis and monitoring for fermentation L.A.B. reactors, and as a fermentation multi-reactor control unit. It is widely used for monitoring of ferments in starter, functional, bio-protective and probiotic cultures. When iCinac is equipped with its external control module (optional), it can control heating baths, peristatic pumps, circulators, and becomes a complete L.A.B. fermentation multi-position bioreactor platform.

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Features

  • Unique solution designed for acidification activity of L.A.B.: simultaneous monitoring of pH, temperature, and Oxidation Reduction Potential (ORP).
  • Uses ISM® protocol digital electrodes (pH/Temp, pH/ORP/Temp)
  • Scalable – up to 32 channels with the wired version, and 16 channels with wireless
  • Meets ISO 26323|IDF 213, the industry standard for determining dairy cultures’ acidification activity
  • Each electrode or channel is independently monitored while providing full control and insight into each experiment
  • Easy-to-use software – results in real-time automatic configuration and detection of connected modules

Benefits

  • Ensures inter-batch kinetics consistency in compliance with ISO standards
  • Reduces product development timelines because of the reactor-scale, efficient design and your choice of ferment
  • Allows you to adapt the configuration to your needs (current and future)
  • ~Wired or wireless version (or combined); select the number of channels, the number of electrodes, and subsequently add or remove electrodes from the systems 
  • Improves quality control, R&D, product development and process optimization
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Applications

The iCinac Series is used in many industries, and monitors the acidification activity of ferments and control of L.A.B. fermentation reactors in:

  • Dairy
  • Fermented foods (L.A.B.)
  • Probiotics
  • Ferment and bacterial culture production

The iCinac is also a useful tool in R&D, product and process development and quality control to:

  • Find the most efficient starter for manufacturing needs
  • Check batch consistency of the starter
  • Determine the acid forming characteristics specific to the phylum studied
  • Provide the insight needed for precise development, test, and process control of fermentation ingredients
  • Define and control the appropriate inocula by characterizing the acid formation of a specific phylum and knowing the specific test parameters’ influence
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iCinac L.A.B. Fermentation Monitor Series

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Product Lineup

iCinac Wireless L.A.B. Fermentation Monitor

iCinac Wireless

  • Up to 60 meter range for indoor use without cables
  • Quick startup, ready for measurement with less than one minute warm up time
  • Scalable from 1 to 16 channels per receiver
  • Lithium-ion rechargeable batteries
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iCinac Wired L.A.B. Fermentation Monitor

iCinac Wired Version

  • 16 or 32 measurement channels
  • 5 or 10m BNC cables to connect each probe
  • Integrated touch screen software
  • Optional output assembly and accessories to control external equipment or conditions (Bioreactor controller)
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How It Works

The Lactic Acid Bacteria

Lactic Acid Bacteria (L.A.B.)’s main function is to produce Lactic Acid. Additionally, L.A.B. contributes to the flavor, texture, and nutritional value of fermented foods through production of aroma components. L.A.B. is also used as adjunct cultures, production or degradation of exopolysaccharides, lipids and proteins, production of nutritional components such as vitamins, and used as functional cultures. L.A.B. promotes therapeutic effects and is used for probiotics. In addition, L.A.B. contributes to the inhibition of spoilage and pathogenic microorganisms and thus, is used as bio-protective cultures.

iCinac Operation

The iCinac, when used as a L.A.B. activity and kinetics monitoring system, works by measuring pH and temperature in each reactor cell and this is represented as a pH vs. time, Temperature vs. Time, and ORP vs. Time series of graphs. Users can then:

  • Compare the curve (kinetics) to their defined Gold Standard or specific kinetics to see if the tested batch behaves as expected – e.g. as a quality control step, in compliance with ISO26323
  • Analyze new ferment strains kinetics and behavior under specific conditions  
  • Develop inter comparison studies between batches, strains, external conditions, and more
iCinac or wireless iCinac configurations for L.A.B. activity and kinetics monitoring


The iCinac, when used as a Fermentation L.A.B. bioreactor controller, not only monitors and records pH, Temperature and ORP (optional) in each reactor, but it also controls external systems through digital and analog outputs, such as peristaltic pumps, heating circulators, and others based on time and pH values. This allows the user to have a reactor that follows not only specific temperature-based programs, but also have automated adjustment of pH values as per the pre-programmed pH kinetics. It ensures specific kinetics and behaviors are followed within each reactor, hence converting iCinac into a laboratory and pilot scale multi-bioreactor platform with complete monitoring, recording and control of external systems.

iCinac used for L.A.B. Fermentation bioreactor control
(example with peristaltic pumps and heating bath recirculators)

A Simple Test, Without Much Preparation

Experiments can be easily set up for iCinac wired:

  1. Turn on the iCinac
  2. Connect all required cables and electrodes
  3. Perform the calibration of all electrodes (pH calibration should be run before each experiment as per the defined protocol)
  4. Insert the electrode in the dedicated bioreactor and adjust the bioreactor conditions
  5. Start the iCinac experiment and initiate to obtain real-time data via the built-in PC
  6. Analyze your data at the end of the experiment
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System Components

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Software

The iCinac software license allows you to install it on multiple computers. This gives you the possibility to retrieve data from your PC while the iCinac is occupied with measurements. In addition, the iCinac software enables you to send emails or SMS to inform users of specific events (reached pH, end of run, alarms, etc.)

The iCinac software license allows you to install it on multiple computers. This gives you the possibility to retrieve data from your PC while the iCinac is occupied with measurements. In addition, the iCinac software enables you to send emails or SMS to inform users of specific events (reached pH, end of run, alarms, etc.)

The iCinac software license allows you to install it on multiple computers. This gives you the possibility to retrieve data from your PC while the iCinac is occupied with measurements. In addition, the iCinac software enables you to send emails or SMS to inform users of specific events (reached pH, end of run, alarms, etc.)

The iCinac software license allows you to install it on multiple computers. This gives you the possibility to retrieve data from your PC while the iCinac is occupied with measurements. In addition, the iCinac software enables you to send emails or SMS to inform users of specific events (reached pH, end of run, alarms, etc.)

The iCinac software interface is intuitive and user friendly. 

Features:

  • pH/ORP temperature compensation management
  • Monitor and track trial set points
  • Control external modules (digital / analog output)
  • Calculate all feature points
  • Real time data logging
  • Diverse graphical plotting
  • pH calibration history
  • Libraries management and creation (average curves, standard deviation, etc.)

The iCinac software interface is intuitive and user friendly. 

Features:

  • pH/ORP temperature compensation management
  • Monitor and track trial set points
  • Control external modules (digital / analog output)
  • Calculate all feature points
  • Real time data logging
  • Diverse graphical plotting
  • pH calibration history
  • Libraries management and creation (average curves, standard deviation, etc.)

The iCinac software interface is intuitive and user friendly. 

Features:

  • pH/ORP temperature compensation management
  • Monitor and track trial set points
  • Control external modules (digital / analog output)
  • Calculate all feature points
  • Real time data logging
  • Diverse graphical plotting
  • pH calibration history
  • Libraries management and creation (average curves, standard deviation, etc.)

The iCinac software interface is intuitive and user friendly. 

Features:

  • pH/ORP temperature compensation management
  • Monitor and track trial set points
  • Control external modules (digital / analog output)
  • Calculate all feature points
  • Real time data logging
  • Diverse graphical plotting
  • pH calibration history
  • Libraries management and creation (average curves, standard deviation, etc.)

By programming a thermal cycle, users can reproduce or simulate the changes in process conditions (e.g., variations in temperature or pH) and use the graphs or descriptors to compare the potential effects on the phylum studied.

Each channel is shown in an independent window.

By programming a thermal cycle, users can reproduce or simulate the changes in process conditions (e.g., variations in temperature or pH) and use the graphs or descriptors to compare the potential effects on the phylum studied.

Each channel is shown in an independent window.

By programming a thermal cycle, users can reproduce or simulate the changes in process conditions (e.g., variations in temperature or pH) and use the graphs or descriptors to compare the potential effects on the phylum studied.

Each channel is shown in an independent window.

By programming a thermal cycle, users can reproduce or simulate the changes in process conditions (e.g., variations in temperature or pH) and use the graphs or descriptors to compare the potential effects on the phylum studied.

Each channel is shown in an independent window.

The iCinac software can perform complex multivariate analyses of the trial curves in order to extract key characteristics of the curve associated with the kinetics of the fermentation. Feature points are useful in comparing the effects of variations in standard conditions on the overall acidification kinetics. Most common feature points for the determination of acidification activity are automatically programmed within the software, but a variety of feature points are user-customizable in order to automatically extract the most useful data from the specific fermentation curves being analyzed.

The iCinac software can perform complex multivariate analyses of the trial curves in order to extract key characteristics of the curve associated with the kinetics of the fermentation. Feature points are useful in comparing the effects of variations in standard conditions on the overall acidification kinetics. Most common feature points for the determination of acidification activity are automatically programmed within the software, but a variety of feature points are user-customizable in order to automatically extract the most useful data from the specific fermentation curves being analyzed.

The iCinac software can perform complex multivariate analyses of the trial curves in order to extract key characteristics of the curve associated with the kinetics of the fermentation. Feature points are useful in comparing the effects of variations in standard conditions on the overall acidification kinetics. Most common feature points for the determination of acidification activity are automatically programmed within the software, but a variety of feature points are user-customizable in order to automatically extract the most useful data from the specific fermentation curves being analyzed.

The iCinac software can perform complex multivariate analyses of the trial curves in order to extract key characteristics of the curve associated with the kinetics of the fermentation. Feature points are useful in comparing the effects of variations in standard conditions on the overall acidification kinetics. Most common feature points for the determination of acidification activity are automatically programmed within the software, but a variety of feature points are user-customizable in order to automatically extract the most useful data from the specific fermentation curves being analyzed.

Real-time graphical presentation of data and integrated pre-programmed data analysis capabilities display the key characteristics of the studied strain without additional user input. Users can create average curves to build a reference database to overlay and compare with new trials. And, personalized profiles can be created for ultimate characterization of the trial activity.

Real-time graphical presentation of data and integrated pre-programmed data analysis capabilities display the key characteristics of the studied strain without additional user input. Users can create average curves to build a reference database to overlay and compare with new trials. And, personalized profiles can be created for ultimate characterization of the trial activity.

Real-time graphical presentation of data and integrated pre-programmed data analysis capabilities display the key characteristics of the studied strain without additional user input. Users can create average curves to build a reference database to overlay and compare with new trials. And, personalized profiles can be created for ultimate characterization of the trial activity.

Real-time graphical presentation of data and integrated pre-programmed data analysis capabilities display the key characteristics of the studied strain without additional user input. Users can create average curves to build a reference database to overlay and compare with new trials. And, personalized profiles can be created for ultimate characterization of the trial activity.

Tabular display of feature acidification characteristics for each curve can be automatically extracted from the raw data for simplified data analysis.


Tabular display of feature acidification characteristics for each curve can be automatically extracted from the raw data for simplified data analysis.


Tabular display of feature acidification characteristics for each curve can be automatically extracted from the raw data for simplified data analysis.


Tabular display of feature acidification characteristics for each curve can be automatically extracted from the raw data for simplified data analysis.


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Installation Options

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Accessories

iCinac L.A.B. Fermentation Monitor Series

Available Accessories

  • Base required to house individual analog and digital output modules
  • Optional iCinac digital output module. 4 outputs (green)
  • Optional iCinac analog output module. 2 outputs (blue). 4-20 mA
  • Optional iCinac analog output module. 2 outputs (red). 0-10VDC
  • Input temperature module for PT100
  • PT100 temperature electrode
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Specifications

iCinac Wired

Weight 11 to 13 kg depending on layout (24.25lbs to 28.7lbs)
Dimensions 510mm L x 450mm W x 300mm H (20” x 17.7” x 11.8”)
Power 12VDC 96W
System Probes InLab® Smart Pro-ISM probe for iCinac (pH/ORP/temp)
InLab® Smart Basic ISM probe for iCinac (pH/temp)

iCinac Wireless

Transmitter Battery 25-watt-hour rechargeable Li-ion
Time Between Charges Typically, 25 days under normal operation
Battery Charge Time Approximately 5 hours (2 hours to 60%) with optional charger
Transmitter Dimensions 52mm diameter x 133mm length (2” x 5.2”)
Wireless Range up to 60 meters (indoors)
Minimum PC Requirements Windows® 10 OS, Intel® Core™ i5, 8GB RAM, 2 USB ports
Compliance EMC EN 61326-1 and FCC Part 15; Safety EN 61010-1
System Probes InLab® Smart Pro-ISM probe for iCinac (pH/ORP/temp)
InLab® Smart Basic ISM probe for iCinac (pH/temp)
System Components iCinac Wireless transmitter with rechargeable Li-ion battery. 1 to 16 transmitters per receiver and license.
iCinac Wireless receiver, software, and license
Optional iCinac wireless charger for up to five transmitters
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